Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAALLGVNIDHVATLRQARGTAYPDPVEAALICEQAGAEGITLHLREDRRHIQDDDVRRMRPLLKTRMNLEMAVTDEMVEFAKEIQPQHVCFVPERRQEVTTEGGLDVVGNFEKVKAATQALAAIGCDVSLFIDADFAQIDAAIACGAPTIELHTGAYTDATTEQEQQEELARIVKGVEYAASKGLVVNAGHGLNLKNIAPIAAIPQIHALNIGHSIIAESVFVGLVQAVKDMKTAIQAAG 1IXN Chain:D ((4-234)) ---LLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVTTEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYADAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAIIGRAVMTGLKDAVAEMK-------

General information: TITO was launched using: RESULT: Template: 1IXN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1333 -170155 -127.65 -736.60 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain D : 0.89 3D Compatibility (PKB) : -127.65 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_1IXN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IXN-query.scw PDB file : Tito_Scwrl_1IXN.pdb: