Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNKYKEVLCVAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHYIRILPGDKVTVELSPYDL--NRGRITYRFK 2NCH Chain:A ((4-78)) ---------DEKSKTLFEVEGAVTALLPAAEFRVKLDNEHEIICHVSGKVRRSKIRIIIGDRVLVEMSIYDRNAKKGRIIRRLK

General information: TITO was launched using: RESULT: Template: 2NCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 284 -38074 -134.06 -521.55 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -134.06 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_2NCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NCH-query.scw PDB file : Tito_Scwrl_2NCH.pdb: