Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MIKGAIFLRPKQQLFITLLVILGA-LFNNISVS---ASAFN---FSVTPITSENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKNEI---EKDMSLIFDLVDY-VSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGIT-----FQEIQTEKDQTETKDQSLSIEN--EYTYIIALLMQQTLNEVAPNLTLHEVKPDQINAR---------NVILANVQNDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYHLTMTVLGNENENGKFS---RKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKD-------NNWIYILVASLFLLFILLCILLFVLRKKEEKEK------ 1O75 Chain:A ((7-414)) ETSYGYATLSYADYWAGELGQSRDVLLADLDAGMFDAVSRATHGHGAFRQQFQYAVEVLGEKVLSKQETE-----DSRGRKKWEYETDPSVTKMVRASASFQDLGEDGEIKFEAVEGAVALADRASSFMVDSEEYKITNVKVHGMKFVPVAVPHELKGIAKEKFHFVEDSRVTENTNGLKTMLTEDSFSARKVSSMESPHDLVVDTVGTGYHSRFGSDAEASVMLKRADGSELSHREFIDYVMNFNTVRYDYYGDDASYTN--LMASYGTK--HSADSWWKTGRVPRISCGINYGF-DRFKGSG--PGYYRLTLIANGYRDVVADVRFLPKYEGNIDIGLKG-----KVLTIGGADAETLMDAAVDVFADGQPKLVSDQAVSLGQNVLSADFTPGTEYTVEVRFKEFGSVRAKVVA

General information: TITO was launched using: RESULT: Template: 1O75.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1776 97630 54.97 280.55 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 54.97 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_1O75.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O75-query.scw PDB file : Tito_Scwrl_1O75.pdb: