Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKPVDVVHNCWGNLFNKNSQNKHIDEKIDYNKQSEHEDQRRENMNIKIISVGK-LKEKYLIQGINEYVKRLN--AYAKIELIEVPDEKAPENLSEAQ------MRQVKEKEG-----ERILAKI----KEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSHLVFVIGGSLGLSEAVMK-RSNAQISFGKMTYP-HQLMRLILVEQIYRAFRINAGAPYHK 4H3Z Chain:B ((20-181)) ------------------------------------------GSMQFDIVTLFPDMFRALTDWGI---TSRAAKQERYGLRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLARPLEDAINAAKAAQAEQGIGGARVVMMSPQGATLNHD-KVMRFAA-----EPGLILLCGRYEAIDQRLIDRVVDEEVSLGDFVLSGGELPAMALIDAVVRHL----------

General information: TITO was launched using: RESULT: Template: 4H3Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 587 12605 21.47 88.77 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 21.47 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_4H3Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H3Z-query.scw PDB file : Tito_Scwrl_4H3Z.pdb: