Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISMKVVFHIDEL--E--KWSETGKNVKNLIKASPETTI-VVSVNGIAITGYLDSAN--------AEF---LDLQ-GVVFHACANAMRANHISES-----------SLPEQVIVVPAGVLDLVELQSQGYAYIKP 2HYB Chain:J ((3001-3127)) ---MKFALQINEGPYQHQASDSAYQFAKAALEK--GHEIFRVFFYHDGVNNSTRLTTPPQDDRHIVNRWAELAEQYELDMVVCVAAAQRRGIVDEGEASRNGKDATNIHPKFRI--SGLGQLVEAAIQADRLVV-

General information: TITO was launched using: RESULT: Template: 2HYB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 373 -12253 -32.85 -123.77 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain J : 0.65 3D Compatibility (PKB) : -32.85 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_2HYB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HYB-query.scw PDB file : Tito_Scwrl_2HYB.pdb: