Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTKCFIINLPYEFTKFYFVCFHHKSKLRFLNYIESTNTILLIKGVFFMTILVLGGAGYIGSHAVDQLVQK-GYQVAVVDNLLTGHKQAVH-------------------PDAHFYE----GDIRDKEFLRSVFEKE-PIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLEVMHEFNVNKIVFSSTAATYGEP---KESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYGMRYVALRYFNVAGAKADASIGEDHTPETHLVPIIL-----QVALGQR-KALAVYGDDYDTPDGTCIRDYVQVEDLIAAHILALEYLKE---GNESNFF---NLGSSKGYSVKEMLEAAREVTGKEIPAEIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE 1GY8 Chain:B ((3-382)) ------------------------------------------------MRVLVCGGAGYIGSHFVRALLRDTNHSVVIVDSLVGTHGKSDHVETRENVARKLQQSDGPKPPWADRYAALEVGDVRNEDFLNGVFTRHGPIDAVVHMCAFLAVGESVRDPLKYYDNNVVGILRLLQAMLLHKCDKIIFSSSAAIFGNPTMTNAEPIDINAKKSPESPYGESKLIAERMIRDCAEAYGIKGICLRYFNACGAHEDGDIGEHYQGSTHLIPIILGRVMSDIA----DKRMPIFGTDYPTPDGTCVRDYVHVCDLASAHILALDYVEKLGPNDKSKYFSVFNLGTSRGYSVREVIEVARKTTGHPIPVRECGRREGDPAYLVAASDKAREVLGWKPKYDTLEAIMETSWKFQRTHPNGY--

General information: TITO was launched using: RESULT: Template: 1GY8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1688 -22721 -13.46 -70.34 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -13.46 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_1GY8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GY8-query.scw PDB file : Tito_Scwrl_1GY8.pdb: