Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNHTRKLLGLTDKHLFFD--EEWL-----IEKEYKGVQAQFIKGKLDDSSSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDE--LLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKQFHLRYLSRLKRNSRSSIATNRVFQMPLRCPTQTASLRG 5JP6 Chain:A ((23-379)) MSQIGSSVRQAVSDNQSAQTLVEWENSEANPEALFANWRHEFMVDSSKRESMKTELCKELQALP------AQDLTLFENEIR---DENNRALVSGCKEELLAQ---VDEHFDEQRESMSVPGHALKAVQSRN----SFRFPDNTQKRDMSNGYMAVRGDVARKEVVLTFDD--GPHGLYTDAILRALKEVNAKAMFFATGKSVRTNPEALKRVAADGHVIGSH---SITLTFDEAAAEVRGGHQAVFDVLGWVDPVFRFYGETSKDLKAFLKTKSTGEFA--------WNIESDDWRTQS-----NEQLLARVLANVESQGRGIVLFHDIQRRTAEIMPQFLRELYNR----GY----------SVVLLTAADPSAKYNSKLVK------------RK

General information: TITO was launched using: RESULT: Template: 5JP6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1794 40097 22.35 121.88 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 22.35 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.188

(partial model without unconserved sides chains): PDB file : Tito_5JP6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JP6-query.scw PDB file : Tito_Scwrl_5JP6.pdb: