Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKILLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGA-EKREKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQLIKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETASEMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQTEYDQAVNVPVTDGLQELTQ------SDDNTKIVDNYVKEVINEVQEKT------DKNVYTDGLEIYTNLDLDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLAVNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAP-YNYEGTSTPVGNWDNQYMGTITLRQALYLSRNVPAVKLFNEV----GSDKVASFLKNLGIEYS-TIHQSNAISSNTEEQDGTKYGASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDILKDTIT-EGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSISVWTGYNKKMTP----V-TSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQYTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSEPASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR 2OLV Chain:A ((9-611)) ---------------------------------------------------------------------------AKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYEHGALDYKRLFGAIGK--------GASTLTQQVVKDAFLS---QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFY--KNKDQQVGATILDSKTGGLVAISGGRDFKDV--VNRNQATD-PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVD--GSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSAS-------EFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQQEYPQFLYENVMSKISSR-DGEDFKRPSSV---------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2OLV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3085 183319 59.42 322.18 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 59.42 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_2OLV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OLV-query.scw PDB file : Tito_Scwrl_2OLV.pdb: