Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEEYEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLEYVQQIQAIEE-- 4RS8 Chain:A ((9-92)) ----------------YIFLTPRAYIIVHLLKVGKAKASEISENTQIPYQTVIQNIRWLLA------------EGYVVKEQKGEEIYYKLTDKG--KQMATAELEKIRKLVEVV

General information: TITO was launched using: RESULT: Template: 4RS8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 267 -27709 -103.78 -337.91 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -103.78 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_4RS8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RS8-query.scw PDB file : Tito_Scwrl_4RS8.pdb: