Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEGQWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNGEDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKENPKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGIE-AREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQEYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGDLIEELIQLGMNRYHQRQSFFEKNE 2ZDH Chain:B ((1-319)) MRVLLIAGGVSPEHEVSLLSAEGVLRHIPF---PTDLAVIAQDGRW----LLGEK--ALTALEAKAAPEGEHP-------FPPPLSWERYDVVFPLLHGRFGEDGTVQGFLELLGKPYVGAGVAASALCMDKDLSKRVLAQAGVPVVPWVAVRKGEPPVVPFDPPFFV----------KPANTGSSVGISRVERFQDLEAALALAFRYDEKAVVEKALSPVRELEVGVLGNVFGEASPVGEVRYEAPFY-------PGRAELLIPAPLDPGTQETVQELALKAYKVLGVRGMARVDFFLA-EGELYLNELNTIPGFTPTSMYPRLFEAGGVAYPELLRRLVELALT-------------

General information: TITO was launched using: RESULT: Template: 2ZDH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1790 -85427 -47.72 -274.68 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -47.72 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2ZDH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZDH-query.scw PDB file : Tito_Scwrl_2ZDH.pdb: