Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGINIENASFGLTNCAERTAFFKAVSEGEK--TFTHLVVAGHTPDPISPCGACRQVMAEFCSPDMPVTLVGDNGVTKATTVRALLPYAFTEKDL 3DMO Chain:D ((28-135)) ---------------------PYSNFKVGAALVTNDGKVFHGCNVENASYGLCNCAERTALFSALAAGYRPGEFAAIAVVGETHGPIAPCGACRQVMIELGKPTLEVVLTNMQGDVRVTSAGDLLPDAF-----

General information: TITO was launched using: RESULT: Template: 3DMO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 502 -19995 -39.83 -188.63 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : -39.83 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_3DMO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DMO-query.scw PDB file : Tito_Scwrl_3DMO.pdb: