Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVEWRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHVIHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVMEHFGADIGKLAVTPNNERDVLRLMELT-RRANAFVSMPIITMSMGNLGKISRLVGGTTGSVMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL 4GFS Chain:B ((7-254)) -VTVRDLVVGEGAPKIIVSLMGKTITDVKSEALAYREADFDILEWRVDHFANVTTAESVLEAAGAIREIITDKPLLFTFRSAKEGGEQALTTGQYIDLNRAAVDSGLVDMIDLELFTGDDEVKATVGYAHQHNVAVIMSNHDFHKTPAAEEIVQRLRKMQELGADIPKIAVMPQTKADVLTLLTATVEMQERYADRPIITMSMSKTGVISRLAGEVFGSAATFGAVK-----APGQISVADLRTVLTILHQ--

General information: TITO was launched using: RESULT: Template: 4GFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1338 -125829 -94.04 -515.69 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -94.04 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_4GFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GFS-query.scw PDB file : Tito_Scwrl_4GFS.pdb: