Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFENKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPEYANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLPKQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVYYDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNEPLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVFALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLERIIPNMEAYFSDYF 2I00 Chain:B ((11-406)) ----------TLKPVEEEHIDQFNELLSYVFQVTEADIEESGFENKRAFIKSKQPILELSKVFGWFHENQLISQIAIYPCEVNIHGALYKMGGVTGVGTYPEYANHGLMKDLIQTALEEMRQDKQWISYLFPYNIPYYRRKGWEIMSDKLSFKIRDTQLPKTVPVPGMIERLAVDHPDVFDVYARFARQNHGALIRSAFNWEEYWRFENEEERTAAVYYGANQEPLGVLFYWVADEVFHIKEMFYLNQEARNGLWNFITAHFSMVYWVKGDIYKNEPLAFLLEDSQIKESIEPYYMARIVDVKAFLENFPFESTAKPFHFVVKDPVAEWNNGIFGLIWDENDQVTITDEPLGTAVHLDIQTLTCLVMNYRRPSYLHRIERIDTDKETLNSLERIFPDQEAYFSDYF

General information: TITO was launched using: RESULT: Template: 2I00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2149 -257910 -120.01 -651.29 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.96 3D Compatibility (PKB) : -120.01 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_2I00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I00-query.scw PDB file : Tito_Scwrl_2I00.pdb: