Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGIMGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLVFPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWLDQVIFSNNPLELVNELRAVSIKNQLLLKEAK 2A33 Chain:B ((14-190)) RRICVFCGSSQGKKSSYQDAAVDLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGRHVIGIIPK--------GETVGEVRAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVITWAQLGI-HDKPVGLLNVDGYYNSLLSFIDKAVEEGFISPTAREIIVSAPTAKELVKKLEE-------------

General information: TITO was launched using: RESULT: Template: 2A33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 892 -122963 -137.85 -727.59 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -137.85 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.588

(partial model without unconserved sides chains): PDB file : Tito_2A33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A33-query.scw PDB file : Tito_Scwrl_2A33.pdb: