Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNTEINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPILNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATIEKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKEQLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKYNTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTTADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDATLKGTND-----DGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVMKELFGMVEKGTPVLVF 4XVO Chain:C ((95-222)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AEVLGVADIDAHTFTVSVDGEVLRKMPASMGKPKFPTPRGTFTALAKEPVVVMDSRTIGIPLSDPEGYKLTVNHAVRVTWGGVYVHSAPWS--VGSQG-YANVSHGCINLSPDNAAWYYDMVSVGDPIIVQ

General information: TITO was launched using: RESULT: Template: 4XVO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 582 -3668 -6.30 -29.82 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.58 3D Compatibility (PKB) : -6.30 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_4XVO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XVO-query.scw PDB file : Tito_Scwrl_4XVO.pdb: