Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREMPFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVR--SAREGELVFANEPLIQVEGPLAHCQLVETALLNMVNF------QTLIATK---------AARIKSVIG-----EDPLLEFGTRRAQELDAAVW--GTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYG--------NDYEAFMAYAKTH-K-DCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVREQLDAAGFTEA--KIYASNDLDEATILNLKM---QKAKIDVWGVGTKLITAYD-QPALGAVFKLVSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEEAIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEEYKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA 3OS4 Chain:B ((6-368)) ----SPILTSLLDTDAYKLHMQQAVFHHYR-HITVAAEFRCRSDELL--GVYADEIRHQVTLMGQLALTSDEFIYLSSLPFFQDDYLHWLRDFRFKPEQVSVAVHDGK------LDIRIAGLWCEVIMWEVPLLAVISEIVHRRRSTQVTTDQAVQQLRTKLEQFNALSADIDITHFKLMDFGTRRRFSREIQHTVVSTLKDEFPYLVGTSNYDLARTLALAPVGTQAHEWFQAHQQISPTLANSQRVALQVWLDEYPNQLGIALTDCITMDA-FLRDF-DLAF--A--NRYQGLRHDSGDPIEWGEKAIAHYEKLGIDPMKKVLVFSDNLDLEKALFLYRHFYQRI-KLVFGIGTRLTCDIPDVKPLNIVIKLVECND-----KPVA----------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3OS4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1482 -76647 -51.72 -237.30 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -51.72 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_3OS4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OS4-query.scw PDB file : Tito_Scwrl_3OS4.pdb: