Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVGYLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL 5US5 Chain:A ((1-87)) MGFTDETVRFDFDDNRKKAISETLETVYRALEEKGYNPINQIVGYLLSGDPAYIPRYQDARNLIRRHERDEIMEELTKYYLANHGID-

General information: TITO was launched using: RESULT: Template: 5US5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 237 -23683 -99.93 -272.21 target 2D structure prediction score : 0.86 Monomeric hydrophicity matching model chain A : 0.93 3D Compatibility (PKB) : -99.93 2D Compatibility (Sec. Struct. Predict.) : 0.86 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.638

(partial model without unconserved sides chains): PDB file : Tito_5US5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5US5-query.scw PDB file : Tito_Scwrl_5US5.pdb: