Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNPATQDAAAAEEESPVVCTAKKYIFDLHSYAWKESDCLIRIPRPHKGMGHGGMRVCYAVEDVDEEGAGTPMVAKMFRRNISDVVEKD-YFNEGAAQCMCEEFANNFNRIHLTNIHKP--NISFLQCYVVRIPRDSIPVASQKKRTGFFSYKTQDTGEVMFVMEPMLSGKFTKYNSNYGETYREDKKAALTSSEAKRRTEVFEAAEAFSHFTLVESGGSMLVCDLQGVNDFLTDPQIHTEDGKGLGMGNMGQEGIDKWIEKHECNEICRALGLQPLLGAVPSSTACAKEENLNLGLRAQLQSQNPVRLRDLVPLTKPLEEMTEAERIEYALKVSRLTD 5E9E Chain:A ((105-276)) --------------------------------------------------------------------GTKFVLKLYKKE--QQASRELYFEDVKMQMVCRDWGNKFNQ------KKPPKKIEFLMSWVVEL-IDRSPSSNGQ--------------PILCSIEPLLVGEFKKNNSNYG--------AVLTN----RST-----PQAFSHFTYELSNKQMIVVDIQGVDDLYTDPQIHTPDGKGFGLGNLGKAGINKFITTHKCNAVCALLDLDVKLG------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5E9E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 805 11057 13.73 66.21 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 13.73 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_5E9E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E9E-query.scw PDB file : Tito_Scwrl_5E9E.pdb: