Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence EDGCSASILKYDANGGQETYYGSALPANGSIAFQPTEFSTEVVISVDANC-----NPTNTDDVKKSVPKGWGLHVYKKVTAIAGES 2CIR Chain:A ((121-166)) ----------------------------------PMDYLLILTVELGSDYLKTAIEVENTSS-SKELKFNWLFHTYFRIED-----

General information: TITO was launched using: RESULT: Template: 2CIR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 76 6823 89.78 166.41 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 89.78 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_2CIR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CIR-query.scw PDB file : Tito_Scwrl_2CIR.pdb: