Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFEKIIQLKNCRYDYTLSPSVKKFTLK--------------DNTFFETKVGNYELTRLLEKVPNS---GEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMDSLVERGVFTKSER 3T2L Chain:A ((152-225)) ----SLVELKISRENYFMPCEISKITLKNSNLIKKGTINIAVDGSIHSSETGNYDLTTVTDASPHTLSVGESYVCRVL------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3T2L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 97 1292 13.32 22.67 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 13.32 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_3T2L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T2L-query.scw PDB file : Tito_Scwrl_3T2L.pdb: