Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLPNPVLAKVTERVIARSQKTRSAYLQRIEHAQGKFPARGALSCANLAHGFAGMEDNEKLIIKVGREPNIGIVSSYNEMLSAHAPYKTFPDIIKTAARENGGVAQFAGGVPAMCDGITQGNASMELSLFSRETIAMGTAIALSHNMFDAALCLGVCDKIVPGLLIGALQFGYLPTIFVPAGPMSSGLSNDDKA-------KIRQQFATGQVGRDALLEAESAAYHGQGTCTFYGTANSNQMLMEVMGLHLPSAAFVHPHTPLRDALTAEAAKRVLDLTAEQGNYTPIGHVIDEKAIINGIVALLATGGSTNHTLHLIAIAHAAGILIDWDDFDELSAVVPLLAKIYPNGKADVNHFQAAGGVAFLIRNLLEAGLLHNDVKTVAGKGLQHYTKEPKLIDGKLTWVDGVVQSLDDKVLRSIDAPFQPDGGLRLMQGRLGR--GVIKISAVAPEHRKVKAPAIVFDSQEAVQAAF--DRGELHRDFIAVVRFQGARAN-GMPELHRLTPVLGVLQDQGFHVALVTDGRMSG-ASGKVPAVIHLSPEALLNGPIGKVQTGDMLIIDAEAGVLDIEL-DEQTWQSRPVAQPEHQAENEVGFGRELFGVFRAAAAPAEHGASVFGALVGEEPQGQI 5J85 Chain:A ((54-548)) --------------------------------------------------------------------PVIGILNTWSDMTPCNGHLRELAEKVKAGVWEAGG---FPLEVPVFS---ASENTFRPTAMMYRNLAALAVEEAIRGQPMDGCVLLVGCD-TTPSLLMGAASCD-LPSIVVTGGPMLNGYFRGERVGSGTHLWKFSEMVKAGEMTQAEFLEAEASMSRSSGTCNTMGTASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLTGRRIVQMVKD--DLKP-SEIMTKQAFENAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGRDVPTIVNLMPSGKYLMEEFFYAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKD--------------VVNWNEDVILPAEKALTSSGGIVVLRGNLAPKGAVLKPSAASPHLLVHKGRAVVFEDIDDYKAKINDDNLDIDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLKKGILDMVRISDARMAGTAYGTV--VLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLAEWQPNH------------------------------------------

General information: TITO was launched using: RESULT: Template: 5J85.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3158 -94471 -29.91 -196.81 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -29.91 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_5J85.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J85-query.scw PDB file : Tito_Scwrl_5J85.pdb: