Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPKSFLIYKNDKLDLCKTLAIIEERLELPSPMEQDDMAGKTLYDKLWDDHVVTQRDDGSCLLYIDRHLLHEVTSPQAFEGLQLAGRQPWRLSANVATPDHNVPTSKKERDQGIAGIEDDTSRIQVQTLDDNCKAFNIVE-FGINDIR---QGIVHVVGPEQGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEVEHVLATQCLVQKKSKNMLVRVDGVLGKG--VTPKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGARVGMVAVDDKTIEYVKGRSYAPKGEQWDQAVAYWNTLHSDDDAVFDAVVELNGAEIEPQVSWGTSPEMVIPVSKAVPTLEQAKDDVQRNDWTRAYQYMGLNAGQALADIQLGRVFIGSCTNSRIEDIRAAAEVVKGRKVAPSIKQAMIVPGSGLVKQQAEKEGLDKIFLEAGFEWREPGCSMCLAMNADKLQPGEHCASTSNRNFEGRQGN-GGRTHLVSPAMAVAAAIAGHFVDIRSF 4KP2 Chain:A ((26-417)) ----------------------------------------------------------------VDLAMTHDGTTPLAYKALKEMSDSVWNPDKIVVAFDHNVPP-------------NTVKAAEMQKL-----ALEFVKRFGIKNFHKGGEGICHQILAEN-YVLPNMFVAGGDSHTCTHGAFGAFATGFGATDMAYIYATGETWIKVPKT--IRVD-IVGKNENVSAKDIVLRVCKEIGRRGATYMAIEYGGEVVKNMDMDGRLTLCNMAIEMGGKTGVIEADEITYDYLK-KERGLSDEDIAKLKKERITVNR-DEANYYKEIEIDITDMEEQVAVPHHPDNVKPIS-----------DVEGT--------------------EINQVFIGSCTNGRLSDLREAAKYLKGREVHKDVK-LIVIPASKKVFLQALKEGIIDIFVKAGAMICTPGCGPCLGAHQGVLAEGEICLSTTNRNFKGRMGHINSYIYLASPKIAAISAVKGYITN----

General information: TITO was launched using: RESULT: Template: 4KP2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2424 37707 15.56 97.94 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 15.56 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.356

(partial model without unconserved sides chains): PDB file : Tito_4KP2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KP2-query.scw PDB file : Tito_Scwrl_4KP2.pdb: