Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIDTQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKLKGK---LFGDRGYVGKNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLR--KRGII-EALFKLMKSQFDLEHSRHRSKMGLLTTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA 2Q1T Chain:A ((26-119)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NASKLANTNVMVVGGAGFVGSNLVKRLLELGVNQVHVVDNLLSAEKINVPDHPAVRFSETSITDDALLASLQDEYD-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Q1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 268 -4741 -17.69 -67.73 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -17.69 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.203

(partial model without unconserved sides chains): PDB file : Tito_2Q1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q1T-query.scw PDB file : Tito_Scwrl_2Q1T.pdb: