Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIAKLKIAIIGLGYVGLPLAVEFGKK-VPVVGFDIYQKRIDELKNGQ----DHTLEVSPEELKQAVHLKYTAHLDDLQDSNFFIVTVPTPIDD--FKQPDLTPLIKASTSIGQVLKKGDVVVYESTVYPGATEEVCIPVLEKISGLKFNQDFFAGYSPERINPGDKQHRVTNILKITSGSTPEVADYIDEVYNLIIEAGTHKAPNIKVAEAAKVIENTQRDVNIALINELALIFNKLDIDTEDVLKAAGTKWNFLPFRPGL-VGGHCIGVDPYYLTHKAQSIGLHPEIILAARRLNDRMGEHVATQLIKEMVKQRIQVV-GARILVMGLSFKENCPDIRNTKIVDFIKAL-KEYDLDLDIYDPWVDDTEVQHEYGLAPIKELQQGLYDA--IVIAVAHNQFKSMSVEEFHALGKEKH-VLYDLKYVLDKESSNIRL 3OJO Chain:A ((13-410)) ------KLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQNGQISIEEPGLQEVYEEVLSSGKLKVST---TPEASDVFIIAVPTPNNDDQYRSCDISLVMRALDSILPFLKKGNTIIVESTIAPKTMDDFVKPVIENL-GFTIGEDIYLVHCPERVLPGKILEELVHNNRIIGGVTKACIEAGKRVYRTFVQ-GEMIETDARTAEMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDP------------NAKLIQTGREINNSMPAYVV-----DTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEH--------DMSHAVKDASLVLILSDHSEFKNLSDSHFD---KMKHKVIFDTKNVV---------

General information: TITO was launched using: RESULT: Template: 3OJO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1957 18591 9.50 49.44 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 9.50 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_3OJO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OJO-query.scw PDB file : Tito_Scwrl_3OJO.pdb: