Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTSKFNQFVRDIYQTNNFIPLHEPRFLGNEKKYVLDTIDSTFVSSVGAYVNDFESKIQHFTGCAKAIATVNGTAALHIALLLAGVKRDDIVITQALTFVATCNALSYIGAEPLFIDVSLKTMGLCPKALDTYLQENAFLDDQGVCKHTATNKRISAIVPMHTFGHPVEIDELQEVVTRWNIALVEDAAESLGSYYKGKHTGIFGLVSALSFNGNKVITTGGGGMVLCQDEELGVRAKHITTTAKIPHPYEFYHDENGFNYRLPNLNAALGCAQMESLEGFLSKKRDLAHQYQNFFKDTDISFFVEPENCQSNYWLNAIICK------NKTQRDL---------------ILDETNSNKVMTRPIWTLMTRLPMYENALQGDLTNSLWLEERVVNIPSSVPLE 1O69 Chain:A ((12-368)) ----------------------------GNELKYIEEVFKSNYIAPLGEFVNRFEQSVKDYSKSENALALNSATAALHLALRVAGVKQDDIVLASSFTFIASVAPICYLKAKPVFIDCD-ETYNIDVDLLKLAIKE---------CE-----KKPKALILTHLYGNAAKMDEIVEICKENDIVLIEDAAEALGSFYKNKALGTFGEFGVYSYNGNKIITTSGGGMLIGKNKEKIEKARFYSTQAR-ENCLHYEHLDYGYNYRLSNVLGAIGVAQMEVLEQRVLKKREIYEWYKEFLGEY-FSFLDELENSRSNRWLSTALINFDKNELNACQKDINISQKNITLHPKISKLIEDLKNKQIETRPLWKAMHTQEVFKGAKAYLNGNSELFFQKGICLPSGTAM-

General information: TITO was launched using: RESULT: Template: 1O69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1894 -184464 -97.39 -549.00 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -97.39 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1O69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O69-query.scw PDB file : Tito_Scwrl_1O69.pdb: