Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSLLGDHLLQRLQQDTEGKLHQRLFRCLRGAIIDGVIQPKTRLPASRDLASEIHVSRNTVLTAYEQLQAEGYLEALTGHGTWVAEKLPESFLNTQNKKKVVSSPNVQSSYALSQRGSNLLGYAAASPHQWGAFVPGAPDVTEFPHHIFSRIQARLSREPDINRLIYSNAGGCIELRSALADYLRVARSVQCDADQIIITEGIHQAIDLVSRALSDMGDKVW-IEDPAYWGMRNTLRINGLDIQPMPVDAEGIIPEENPAVPPKLIFVTPSHQYPLGSHLSLDRRRQLIQIARQH-NSWIVEDDYDSEFRFSGQPYPSLQGLENNAPVLYMGTFSKTIYPSLRIGYLVVPKPLFSPLRIVAAELYRGGHLLEQKALAEFIREGHYEAHIRRMRLLYGKRRDYLVSLIQRYLGPEFIHEYDEAAGLHLVLKLPNSCDDVAIAATALERGVKVRPLSQYYMQSHAHAERGLLMGFACVNEKDMVMAFGVLLQYLREADVPTLN 4TV7 Chain:A ((16-457)) --------------------IYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVEE------LD-------MFSA------ALP----DDLKEIHI----WISFSHM--DTDHFPIKSWFRCEQKAASRSY-----MSHPQGIYEVRAAITRLISLTRGVKCRPEQMIIGAGTQVLMQLLTELLPK--EAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITRQQPDVLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDRFQNVIYMGTFSKSLLPGLRISYMVLPPELLRAYKQRGYDLQTCSSLT-QLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEAEFSGE-VTVKGANAGLHFVTE-DTRRTEQDILSHAAGLQLEIFGMSRF--------RPALIIGFARLKEEDI--------------------

General information: TITO was launched using: RESULT: Template: 4TV7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1942 -92511 -47.64 -224.54 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -47.64 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_4TV7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TV7-query.scw PDB file : Tito_Scwrl_4TV7.pdb: