Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELLTLARPYAKAAFAYASEQGATDNWSNALQVLSAAVQDEAFSAYLNR---PEHTPAEQVKLFAKVLGEDQSQAVSNFLTLLADNDRLVLLPEIAAEYEQLKSQNNNNVDVVIESAFPLTAEQEQLLKSALEKRFNSTVTVSVEVKPELIAGVVIRAGDQVIDDSALNKLEKMRTRLLA 5T4Q Chain:L ((3-162)) --EFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQMAELLSGALAPE-TLAES---FIAVAGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAVSEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNAKIDKSVMAGVIIRAGDMVIDG---------------

General information: TITO was launched using: RESULT: Template: 5T4Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 516 -24436 -47.36 -155.64 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain L : 0.75 3D Compatibility (PKB) : -47.36 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.335

(partial model without unconserved sides chains): PDB file : Tito_5T4Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T4Q-query.scw PDB file : Tito_Scwrl_5T4Q.pdb: