Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATMQCDVVSVKESLYSGAVTMLIAKGAGGELGILPGHAPLVTLLQLGPIRVLLENGTEEIVYVSGGVLEVQPHVVTVLADTAIRADNLDEAAILEARKNAEQLLANQKSDLDSAAALAALAETAAQLETIRKIKNRAQ 5T4O Chain:H ((4-137)) --TYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLTAIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKAEEHISSSHGDVDYAQASAELAKAIAQLRVIELTKK---

General information: TITO was launched using: RESULT: Template: 5T4O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 405 -66793 -164.92 -498.46 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain H : 0.86 3D Compatibility (PKB) : -164.92 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_5T4O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T4O-query.scw PDB file : Tito_Scwrl_5T4O.pdb: