Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRLITRPINKIKQVILDFIHHNHIINWTPLQKSALMLTLACAMNFSWILWKGYILITPSIWQWANLSLVESQIWLNLLTLFLLAVLIIPCYRYKDQAWAQKIVPLISVQAFMLMLCHDGYLIGSISPATMVGYVGTIGVGLVLFERKIVYSAFIPATIILAVCTYLSMTGVISYAPLFNFTAMRHAQTNPFWLGSMLFFIMPILVACFILFEILLIQWRQRETYIQRLSQIDPLTNVLNRRSLNTHLEALHEQQF----DYAVILLDIDHFKKINDIYGHHQGDQVLIEIARCLSENLRNEDIIGRFGGEEFILLLPHTDILQAEKIAERCRQ--ALQELTIFNNQNIQIHVSASFGISSSAFAND-PYLVIRQADQALYAVKASGRNKVRTFHQLPQIKSI 2V0N Chain:A ((287-448)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LAVTDQLTGLHNRRYMTGQLDSLVKRATLGGDPVSALLIDIDFFKKINDTFGHDIGDEVLREFALRLASNVRAIDLPCRYGGEEFVVIMPDTALADALRIAERIRMHVSGSPFTVAHGREM-LNVTISIGVSATAGEGDTPEALLKRADEGVYQAKASGRNAV------------

General information: TITO was launched using: RESULT: Template: 2V0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 697 -15585 -22.36 -100.55 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -22.36 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_2V0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V0N-query.scw PDB file : Tito_Scwrl_2V0N.pdb: