Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNISYELEKKIVNLNALYNEEEAELSLRLSQNIRKNQQDGYVYSLISRGFEIKGLKLWLVDKNIDQLKQWFYVSSVLRAESCKYSSGYTLSTPDEFIFPLLSDNTEIINKFAYLDTVNLLWGEFEPSYQEAKFKP-SKDDPRFRTHALQAVLRNDWEDYKKIKEVANQKINKLREVVQFEFGIYDAIYEKDKDKIIEIVQTLLNPRVHKTYNKDFGEEFNGEIWSHHPVMFTKLAWMNGLEIEIDNPLVPMELMPIKPLEHYDYHYDFLDPNWKPKSLFEKLFGKKRNK 5FXF Chain:A ((362-486)) ---------------------------------------------------------------------------------------------------VLQDRHKINNGIPSLDELQLL--DWVPNGGHIGFSPVSAPDGREAMKQFEMV-RNRANEYNK--DYAAQFIIGLREMHHVCLFIYDTAIPEAREEILQMTKVLVREAAEAGY---------GEYRTHNALMDDVMATFN---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FXF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 464 -7407 -15.96 -59.73 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -15.96 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_5FXF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FXF-query.scw PDB file : Tito_Scwrl_5FXF.pdb: