Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSVLTMAVNDSPITSVKTTQPLIALYCGSRAGNNPIYREKAIELASGIAEQGFGLVYGGASIGLMGQVAESVLAHGGEAVGVIPEFMLDYEVAHAGLTELHVVETMHERKAMMAERASAFIALPGGLGTFEEILEIATWGQLNQHQKPMILYNVNGFYNALIAQLDHAVQEGFLPLQHRAKLIVCEEIDQIYNVIKNLKMPKRFVV 3QUA Chain:A ((21-190)) -------------------RQWAVCVYCAS-GPTHPELLELAAEVGSSIAARGWTLVSGGGNVSAMGAVAQAARAKGGHTVGVIPKALVHRELADVDAAELIVTDTMRERKREMEHRSDAFIALPGGIGTLEEFFEAWTAGYLGMHDKPLILLDPFGHYDGLLTWLRGLVPTGYVSQRAMDSLVVVDNVE-----------------

General information: TITO was launched using: RESULT: Template: 3QUA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 862 -116290 -134.91 -684.06 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -134.91 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_3QUA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QUA-query.scw PDB file : Tito_Scwrl_3QUA.pdb: