Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEPVTAMTTLSNSVSKPDIDDVNIKSIQTLVTPAELKSELPLSDVASQTVLKGRETIRNILDGSDKCLFVVIGPCSIHDPKAAHEYADRLKALSEKVKDTLYLVMRVYFEKPRTTIGWKGLINDPDMNDSFNIEKGLRIGRKLLLDLNEKGLPCATEALDPNSPQYYQDLISWSAIGARTTESQTHREMSSGLSSPVGFKNGTDGGLTVATNAMQSVKYGHSFLGLNEDGQVSIINTKGNPYAHVVLRGGNGKPNYDAGSVAEAENALAKAKVSSKIMIDASHANSNKDPYLQPLVLKNITEQILDGNKSIVGIMVESHLKGGRQDIPDNLCDLEYGKSVTDGCIDWETTEKTLLEMHKALKEVLPNR 1N8F Chain:A ((8-346)) ----------------------LRIKEIKELLPPVALLQKFPATENAANTVAHARKAIHKILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTVGWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWGAIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHSAIVNTSGNGDCHIILRGGK-EPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFKKQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGE-PLAYGKSITDACIGWEDTDALLRQLANAVK------

General information: TITO was launched using: RESULT: Template: 1N8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1935 -155823 -80.53 -459.65 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -80.53 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_1N8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N8F-query.scw PDB file : Tito_Scwrl_1N8F.pdb: