Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGHSVALNFADGKTFFISVNKDELLLDAAVRQGINLPLDCREGVCGTCQGQCETGIYEQEYVDEDALSERDLAERKMLACQTRVKSDAAFYFDHDSAICNAGDTLKIETQVTAVELVSETTAILHLDASNHAEQLQFLPGQYARLQIPDTEDWRSYSFANRPNATNQLQFLIRLLPDGVMSNYLRDRCQVGQSLLIEAPLGSFYLREV-ERPLVFVAGGTGLSAFLGMLDNLVEQPNSPTVQFYYGVNSETDLCEQQRLHAYAEQLPNFSYHPIVTKATETWQGKAGYIHEHLNKDQLAEQAFDMYLCGPPPMIEAVKNWLDEQALQNYRIYSEKFLQSNTSR 4WQM Chain:A ((16-309)) -------------------DSNDTLLSAALRAELVFPYECNSGGCGACKIELLEGEVSNLWPDAPGLAARELRKNRFLACQCKPLSDLKIKVINRAEGRASHPPKRFSTRVVSKRFLSDEMFELRLEAE---QKVVFSPGQYFMVDVPEL-GTRAYSAAN-PVDGNTLTLIVKAVPNGKVSCALAN--ETIETLQLDGPYGLSVLKTADETQSVFIAGGSGIAPMVSMVNTLIAQGYEKPITVFYGSRLEAELEAAETLFGWKENLKLINVSSSVVGNSES-SYPTGYVHEIIPEYMEGLLGAEFYLCGPPQMINSVQKLL----------------------

General information: TITO was launched using: RESULT: Template: 4WQM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1624 -117345 -72.26 -400.49 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -72.26 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_4WQM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WQM-query.scw PDB file : Tito_Scwrl_4WQM.pdb: