Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAPVALTPELLTQLTAIVGENRIKTDADSLENWGKDHTKHFNPNPSVIVFPSTTEQVQEVVKLANQFNIAITPSGGRTGLSAGAV--ATKGEIVISMD--KMNQILEFFPADRMVRVQAGVVTEQLQNYAEEQGMYYPVDFASAGSSQIGGNIGTNAGGIKVIKYGMTRNWVLGLTVVTGKGDILRLNKGMIKNATGYALQHLFIGGEGTLGLVTEAEIKLERQPQNLQVLVLGVPDFDAVMPVLHAFQKD---------IDLTAFEFFGELTMQ-------KVLDRGHVQRPFETQCPFYVLLEFEAPYEPIIDKAMEIF------------EHCMEQGWVLDGVMSQSLDQVESLWRLREDISESIAPFIPYKNDISVLITHVPAFIREIDAIVQENYPYFEICWFGHIGDGNLHLNILKPENLTK-DEFFAKCQVVNKYVFDTVKKYDGSISAEHGVGMTKKPYLEYSRSAEEIEYMKALKKVFDPKGIMNPGKLFDL 4BC7 Chain:A ((202-652)) -----------------------------------------FERIPDIVLWPTCHDDVVKIVNLACKYNLCIIPIGGGTSVSYGLMCPADETRTIISLDTSQMNRILWVDENNLTAHVEAGITGQELERQLKESGYCTGHEPDSLEFSTVGGWISTRASGMKKNIYGNIEDLVVHMKVVTPRGVIEKSCQGP-RMSTGPDIHHFIMGSEGTLGVITEATIKIRPTPEYQKYGSVAFPNFEQGVACLREIAKQRCAPASIHLMDNQQFQFGHALKPQVSSIFTS----------GFDPNQLSVATLLFEGDREKVLQHEKQVYDIAAKFGGLAAGEDNGQRGYLLTYVIAYMRDLGLEYYIIGESF-ETSAPW----DRVVDLCRNVKERIRRECKEKGVQFPPLSTCRVTQTYDAGACIYFYFAFNYRGISDPLAVFEQTEAAAREEILANGGSLSHHHGVGKLRKQWLKESISDVGFGMLKSVKDYVDPTNIF--------

General information: TITO was launched using: RESULT: Template: 4BC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2139 48011 22.45 118.84 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 22.45 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_4BC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BC7-query.scw PDB file : Tito_Scwrl_4BC7.pdb: