Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIEFIATSKLPTAFGEFNISVFQDPVTGEEHVALSKGLENPPTDPVLVRVHSECLTGDAFASLKCDCGPQLQATQKLINEAGQGVILYLRQEGRGIGLTNKIRAYALQDQGHDTVDANLLLNLPADARRYDMCSIMLDHLKVKEVKLITNNPLKIQALKDQGINVVDRVPLTVGRNPFNEQYLKTKRERMAHLYQKDDF 4RL4 Chain:A ((24-193)) --LEVSNQAKLPTQFGEFYIQCFREKGS---KDHLVVFTPNFSQNP-LVRLHSECLTGDALGSQKCDCGGALQMALERISKEG-GLVIYLRQEGRGIGLFNKVNAYALQDKGYDTIQANEMIGFKDDERDYSVAGEILEYYRIKKMRLLTNNPKKIAALEKYA--EVTRESLIVCA------------------------

General information: TITO was launched using: RESULT: Template: 4RL4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 766 -77503 -101.18 -464.09 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -101.18 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_4RL4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RL4-query.scw PDB file : Tito_Scwrl_4RL4.pdb: