Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHLHHQTSQTKLWAVIPAAGSGSRFSKTELKQYQYIQDATVIEHTVKRLSQLP-LTGYVLAIGKQDTFASTLSFQDKHKAHFCNGGVERVHSVLNALNYLSQIADEDDWVLVHDAARPCVTFECLNTLVKNAIETNQSAILAIPVRDTLKQVNQ-EQQIEKTVSRELLWQAQTPQIAKIGILKKAIETALKNNLTITDEASALESIGESVQVVMGRSDNIKITYPDDLELARLILQSQN 1H3M Chain:A ((5-226)) ----------LDVCAVVPAA---------CPKQYLSIGNQTILEHSVHALLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLTGL----KAAGDAQWVLVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGLWHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPEDLALAEFYL----

General information: TITO was launched using: RESULT: Template: 1H3M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1122 -58362 -52.02 -276.59 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -52.02 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_1H3M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H3M-query.scw PDB file : Tito_Scwrl_1H3M.pdb: