Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKGMINRELKREKTVAKYAVKRAELKATIANVNASDEERFEAMLKLQALPRNASPVRLRNRCGLTGRPHGYFRKFGLSRNKLRDTVMQGDVPGVVKASW 5UZ4 Chain:N ((2-99)) -AKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNAVLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKA--

General information: TITO was launched using: RESULT: Template: 5UZ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 265 5037 19.01 51.40 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain N : 0.86 3D Compatibility (PKB) : 19.01 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_5UZ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UZ4-query.scw PDB file : Tito_Scwrl_5UZ4.pdb: