Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLIIGIDPGSRLTGYGIIEKDGS--KLRFVDAGTIRTETQE-MPERLKRIFAGVERIVKFHGPTEAAVEQVFMAQNPDSALKLGQARGAAIAALVNLDLQVAEYTARQIKQSVVGYGAADKEQVQMMVMRLLNLTIKPQ-ADAADALAAAICHAHASGSMSKLTVLNALGGMARGRSRSSSRRR 4LD0 Chain:A ((5-158)) --VVAGIDPGITHLGLGVVAVEG-ALKARLLHGEVVKTSPQEPAKERVGRIHARVLEVLHRFRPEAVAVQEQFFYRQNELAYKVGWALGAVLVAAFEAGVPVYAYGPMQVKQALA------KEEVALMVRGILGLKEAPRPSHLADALAIALTHA------------------------------

General information: TITO was launched using: RESULT: Template: 4LD0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 657 -44130 -67.17 -310.77 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -67.17 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_4LD0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LD0-query.scw PDB file : Tito_Scwrl_4LD0.pdb: