Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQSTGAPSLFDDGQIGTGGNNNSQSQVKLQAINLIDLLHDGFYLIFLIRNQYVPADPQRFREKILDLLNRFEQQAKKLQFSADDIHDAKYAFCALIDETIVTQQDPSYFNLQNSWLISPLQLSLFGSQLAGYQFFEILEQLRSRGKDRLAALEVFHYCLLLGFQGKYRIESIESLNHLVARVGDEIDYLKGKKVAFSPFSAIPDQIRNIIHRELPFFWILIFLLIFALLTFAGLRFMLNNQNDKALSNYQNVISAPQEQAHITIYLP 3OET Chain:A ((123-203)) -------------GIVGVG-NVGSRLQTRLEALGIRTLLCD-------------PPRAARGDEGDFRTLDELVQEADVLTFHTPLYKDGPYKTLHLADETLIRRLKPG----------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3OET.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 267 -20067 -75.16 -247.74 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -75.16 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.048

(partial model without unconserved sides chains): PDB file : Tito_3OET.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OET-query.scw PDB file : Tito_Scwrl_3OET.pdb: