Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPAVKSNRPVNLSMGQVLAVNLRSPV-AIASILHRLSGVIVFLLVPVLLWILDKSLSSPEGFDYVKNVVFGNILVRFVVWVFVAGLIFHFIAGVKHLLADLGFAEE-LQSGRIAATISLILSVVAIIAAFVWIVF 2ACZ Chain:C ((5-119)) ---VKKQRPVNLDLQ-----TIRFPITAIASILHRVSGVITFVAVGILLWLLGTSLSSPEGFEQA-SAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFGYLEETFEAGKRSAKISFVITVV-----------

General information: TITO was launched using: RESULT: Template: 2ACZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 237 -37496 -158.21 -331.82 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -158.21 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_2ACZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ACZ-query.scw PDB file : Tito_Scwrl_2ACZ.pdb: