Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVVAFKQVDVFTSQAFKGNPVAVIMDASTLTSEQMQAIANWTNLSETTFVLPAIDSQADYQVRIFTPQSELPFAGHPTIGTAYALLEAGLVKAKEGQLVQQCAAGLVTLTV---NDSNHISFELPKPKITPLDALQTQKLAEILKCKINTQWN------AALVDVGARWVVLQAVNAKAVLASQPDLNALKQFSLEMKVTGSTIYGFYEESNEQKRIEVRSFAPAYGVNEDPVCGSGNGSVASFIRYHSILPAQNDVML--SSQGQALGREGQLQLEL-----HQDKILVGGAAVTCIDGTIKL 1QY9 Chain:A ((7-286)) -------HVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGHSETAFLLHSDDS--DVRIRYFTPTVEVPICGHATVAAHY--VRAKVLGLGNCTIWQTSLAGKHRVTIEKHNDDYRISLEQGTPGFEP--PLEGETRAAIINALHLTEDDILPGLPIQVATTGHSKVMIPLKPEVDIDALSPDLNALTAISKKIGCNGFFPFQIRPGKNE---TDGRMFSPAIGIVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQPEKVTISGTAV---------

General information: TITO was launched using: RESULT: Template: 1QY9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1508 -20521 -13.61 -77.73 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -13.61 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_1QY9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QY9-query.scw PDB file : Tito_Scwrl_1QY9.pdb: