Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLSVGLKVANSLSLTPQLQQAIRLLQLSGLELEQEIQIQLDSNPLLEKVEDELLAESLSTLEHKETDDLTTELNADHLPDDLPVDTEWDDIYTHQSTALGTPEFEEREDNRQVHLTLKEHILEQVNLLHFSKIDQLIAYCIVDALDDKGFLDAELEEIILAVQHLLSEMDIDEEVEEDEVLVVLKHIQRLDPIGIGARNLAECLKVQLEFLPRETEYLKEARSLLQYYELLIANDLNKLLKQTGLSKEQLKFAVDLLKTLKPYPGMDFEKQESEYQIPDVVVAKKDLHWQVQLNPDVMPKLRINSFYSSMIRRADQSDDNLYLRNQMLEA----KNFIKSIDERHKTLLKVATCIVEHQKAFLEIGPEAMKPLVLRDVAEEVELHESTVSRVTTNKYMLTPRGLFELKYFF--SSHVGTTTGGEASSTAIRAMIKKLVSNENPRKPLSDNAIAALLKEEGIEVARRTVAKYRESLHIPSSSERKVLI 5UI5 Chain:I ((33-342)) --------------------------------------------------------------------------------------------------------------------------------------EQELALELLNYLNEKGFLSKSVEEISDVLRCSVEELE-----------KVRQKVLRLEPLGVCSKDVWEFLELQI-----------------EEIYPEEEEILKKALRDLKRGKKLKPEIKGKLSRLRLFP---SAEKVYTFAKVDAIIEEENGEFFIYLYEDFID-IDLNEEYWELYK---N------LQKELKEAFERYESIRKVLDIRRRNLRKVLEKIVERQKDFL-TGKGSLKPLTLREVSSEIGIHESTLSRIVNSKYVKTPVGTYSLRTFFVRESAEGLTQG------ELMKLIKEIVE----RKPYSDQEIANILKEKGFKVARRTVAKYREMLGIPSSRERRI--

General information: TITO was launched using: RESULT: Template: 5UI5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 1033 -40167 -38.88 -136.62 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain I : 0.70 3D Compatibility (PKB) : -38.88 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_5UI5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UI5-query.scw PDB file : Tito_Scwrl_5UI5.pdb: