Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK-DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK 1HR0 Chain:P ((2-82)) --VKIRLARFGSKHNPHYRIVVTDARRKRDGKYIEKIGYYDPRKTTPDWLKVDVERARYWLSVGAQPTDTARRLLRQAGVFRQ---------

General information: TITO was launched using: RESULT: Template: 1HR0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 344 -9898 -28.77 -123.73 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain P : 0.79 3D Compatibility (PKB) : -28.77 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_1HR0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HR0-query.scw PDB file : Tito_Scwrl_1HR0.pdb: