Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTI----NDYNSIDQVVNV--------------INKLNEKNIETRALWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK 1O69 Chain:A ((12-372)) --------------------------GNELKYIEEVFKSNYIAPLGEFVNRFEQSVKDYSKSEN-ALALNSATAALHLALRVAGVKQDDIVLASSFTFIASVAPICYLKAKPVFIDCD-ETYNIDVDLLKLAIKECEKK---PKALILTHLYGNAAKMDEIVEICKENDIVLIEDAAEALGSFYKNKALGTFGEFGVYSYNGNKIITTSGGGMLIGKNKEKIEKARFYSTQARENCLHYEHLDYGYNYRLSNVLGAIGVAQMEVLEQRVLKKREIYEWYKE-FLG-EYFSFLDELENSRSNRWLSTALINFDKNELNACQKDINISQKNITLHPKISKLIEDLKNKQIETRPLWKAMHTQEVFKGAKAYLNGN----SELFFQKGICLPSGTAMSKDD-------------

General information: TITO was launched using: RESULT: Template: 1O69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1989 -126923 -63.81 -370.04 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -63.81 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_1O69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O69-query.scw PDB file : Tito_Scwrl_1O69.pdb: