Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTAFLFSGQGAQYQGMGKDLYEE-AVVKQTFDEASEILGYDMAELCFTEN-ERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAAL-GLSLGEYSALVASNALSFTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTPQQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFSEPSFPIISNTT----TEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVLSGFMKKIDKTIPVLRVENKQTFDETIAIL 3HJV Chain:A ((8-309)) -KFAIVFPGQGSQAVGMLADLAEQYAVVKQTFAEASEVLGYDLWALVQDGPVEDLNQTFRTQPALLAASVAIWRVWQQLGLEQPAVLAGHSLGEYSALVCAGVIDFKQAIKLVELRGQLMQQAVPAGTGAMYAIIGLEDEAIAKACADAAQGEVVSPVNFNSPGQVVIAGQKDAVERAGVLCKEAGAKRALPLPVSVPSHCALMKPAADELAKTLAELEFNAPQIPVINNVDVVAETDPVK---IKDALIRQLYSPVRWTECVEQMSAQGVEKLIE-GPGKVLTGLTKRIVKTLEGVAVNDVASLD------

General information: TITO was launched using: RESULT: Template: 3HJV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1728 -27639 -15.99 -94.01 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -15.99 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.631

(partial model without unconserved sides chains): PDB file : Tito_3HJV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HJV-query.scw PDB file : Tito_Scwrl_3HJV.pdb: