Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHTTQLYQHVPETRWPIVYSPRYNITFMGLEKLHPFDAGKWGKVINFLKEEKLLSDSMLVEAREASEEDLLVVHTRRYLNELKWSFAVA-----TITEIPPVIFL-PNFLVQRKVLRPLRTQTGGTIMAGKLAVERGWAINVGGGFHHCSSDRGGGFCAYADITLAIKFLFERVEGISRATIIDLDAHQGNGHERDFMDDKRVYIMDVYNR-------HIYPGDRF---AKQAIRRKVELEW---GTEDDEYLDKVERNIKKSLQEHLPDVVVYNAGTDILEGDRLGGLSISPAGIVKRDELVFRMVRGRRVPILMVTSGGYQ-KRTARIIADSILNLFGLGLIGPESPSVSAQNSDTPLLPPAVP 5EEF Chain:A ((29-339)) ----------------------------------HPECPARVSTVMEMLETEGLLGRCVQVEARAVTEDELLLVHTKEYVELMKSTQNMTEEELKTLAEKYDSVYLHPGFFSSACLSVGSVLQLVDKVMTSQL--RNGFSINRPPG-HHAQADKMNGFCMFNNLAIAARYAQKR-HRVQRVLIVDWDVHHGQGIQYIFEEDPSVLYFSVHRYEDGSFWPHLKESDSSSVGSGAGQGYNINLPWNKVGMESGDYITAFQQLLLPVAYEFQPQLVLVAAGFDAVIGDPKGGMQVSPECFSILTHMLKGVAQGR---LVLALEGGYNLQSTAEGVCASMRSL--LGDPCPHLPSSGA-------------

General information: TITO was launched using: RESULT: Template: 5EEF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1688 18093 10.72 62.17 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 10.72 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_5EEF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EEF-query.scw PDB file : Tito_Scwrl_5EEF.pdb: