Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ACSIELLNVNQAVVDTKCIPFDTTTAMKDPTGPGGGQL--YKVHVNNLCGIGLDDGQELANGASLRKLSSP 2KL6 Chain:A ((439-470)) ---------------------DLTVEIKGPDVVGVNKLAEYEVHVKNLGGIGV------------------

General information: TITO was launched using: RESULT: Template: 2KL6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 43 2 0.05 0.07 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 0.05 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_2KL6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KL6-query.scw PDB file : Tito_Scwrl_2KL6.pdb: