Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFHGFITRKQTEQLLRDKALGSFLIRLSDRATGY-ILSYRGSDRCRHFVINQLRNRRYIISGDTQSHSTLAELVHHY 2FO0 Chain:A ((104-179)) WYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESR-FNTLAELVHHH

General information: TITO was launched using: RESULT: Template: 2FO0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 255 -21853 -85.70 -291.37 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -85.70 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_2FO0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FO0-query.scw PDB file : Tito_Scwrl_2FO0.pdb: