Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQMEMQLMAFELVDLDRQGTRMKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSKNPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHE-----W---------QVYKTGIENFETARRLFLENKGELLPNSPIQYETATEMKSRFLECMSKY---REHQTVVVVAHRMLMRQFVPNEKIDFCQVIECELEI 1H2F Chain:A ((4-155)) -----------------------LYLTRHGETKW-NVERRMQ---GWQDSPLTEKGRQDAMRLGKRLEAVELAAIYTSTSGRALETAEIVRGGRLIPIYQDERLREIHLGDWEGKTHDEIRQMDPIAFDHFWQAPHLYAPQRGERF----------CDVQQRALEAVQSIVDRHEGETVLIVTHGVVLK--------------------

General information: TITO was launched using: RESULT: Template: 1H2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 531 -14431 -27.18 -106.90 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -27.18 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_1H2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H2F-query.scw PDB file : Tito_Scwrl_1H2F.pdb: