Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLIYFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLFVQSYLGSNTAWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAIFENLLDSYLLQNQSEVD-LDIFYKVLPLL-YKEKYTMGFNYFVSLNYLLYKVGF---LGIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG 4R04 Chain:A ((917-1024)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KEIFS-KYSEHITKEISTIKNSIITDVNG------QLDHTSQVNTLNAAFFIQSLIDYSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVL---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4R04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 234 -14165 -60.53 -146.03 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.48 3D Compatibility (PKB) : -60.53 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 0.336

(partial model without unconserved sides chains): PDB file : Tito_4R04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R04-query.scw PDB file : Tito_Scwrl_4R04.pdb: